The P2 laboratory is mainly composed of four parts: generally divided into  P2 main laboratory, first changing room, second chaning room and washing room. Considering that the biological laboratory should implement the requirements of clean area and non-clean area at the same time, the clean area is mainly reflected in the layout and maintenance structure of the microbial experiment area, and the non-clean area is mainly reflected in the air conditioning purification system.

In terms of layout, the flow of people and logistics are separated to prevent cross-infection of people and objects, that is, the experimental operator enters the P2 main laboratory through a buffer room and a second buffer room, and the experimental objects are transferred through two interlocking The windows enter and exit the P2 main laboratory. One interlocking transfer window is located between the main laboratory and the preparation room, and the other is located between the main laboratory and the washing room to ensure that the clean objects are not infected and pollution. The objects are cleaned as soon as possible.

1. P2 laboratory decoration

The frame of the main body can be partitioned by color steel plate glass. The burning performance of the color steel plate is non-combustible. The thickness of the partition glass is 5 mm. In order to prevent dust deposition, aluminum alloy arc pressure wires can be used for window materials. The aluminum alloy should be used for the inside of all connections. The inner rounded corners and the exposed connecting wires also need to be connected with aluminum alloy outer rounded corners. The ceiling material can be a color steel plate. The indoor floor is made of epoxy resin, which has no gaps and is also very resistant to corrosion.

2. The basic configuration of P2 laboratory

2.1. The cleanroom needs to be equipped with 2 transfer windows to ensure the cleanliness of laboratory logistics. There is a UV lamp in the transfer window, which can sterilize the contaminated items before taking it out of the laboratory, ensuring the isolation of outdoor and indoor air. The transfer window can be controlled indoors and outdoors, reducing the number of laboratory personnel entering and leaving the laboratory. , Which facilitates the delivery of items for experimenters.
2.2. The automatic door closer shall be installed on the door of the clean laboratory in accordance with national regulations.
2.3. The laboratory of interlocking doors is equipped with a set of two-door interlocking electronic interlocks. When one door is not closed, the other door cannot be opened. This will buffer the airflow into the cleanroom. The locked door is also equipped with an emergency switch. When an accident occurs, press the emergency switch to facilitate laboratory personnel to escape the scene as soon as possible.
2.4. The multi-function controller can be used as the general control switch of the laboratory, including temperature and humidity adjustment and display, the start-stop and start-stop sequence of the blower and exhaust fan, lighting switch, UV lamp control, etc. The temperature adjustment display screen can accurately display the temperature in the laboratory with convenient adjustment and small temperature error.
2.5. The differential pressure gauge needs to be equipped with a differential pressure control system for the P2 laboratory in accordance with the requirements of the biological laboratory specification, which can display the amount of indoor and outdoor differential pressure, and can manually control the differential pressure as required to reach a safe range.
2.6. Sockets According to the different purposes of each laboratory, a sufficient number of single-phase nine-hole sockets and three-phase four-hole sockets should be configured. The sockets are all built-in, air-tight design, and need a dust-proof, waterproof, and leakage-proof combined power strip.
2.7. Human body pyro-infrared sensor In order to facilitate use and save power, an infrared detector is placed at the door of the buffer room to sense human body heat, automatically turn on the fan to send out clean air and automatically turn off after a delay.
2.8. The washing room table is equipped with a washing table, a drip rack, a faucet, the sink and the drain hose are all acid and alkali resistant PP materials.
2.9. Eyewash and sensor faucet must be equipped with an eyewash on the washing table in the microbiological experiment area. When the experimenter’s eyes come into contact with dangerous substances, he can use it to wash his eyes. The sensor faucet can sense body heat, automatically discharge water, and shut down after a delay.
2.10. The sample car is convenient for laboratory personnel to move samples and other experimental supplies. A small sample car is equipped in the washing room. The sample car is a stainless steel structure with two upper and lower floors, which is flexible and compact.
2.11. The utensil trolley is used for utensils to be dried after being cleaned. The cleaned utensils can be placed on a drip trolley and dried with dripping water. There is also a sink under the drip rack for collecting drips.
2.12 The laboratory bench is the necessary equipment in the laboratory. The tabletop needs to be resistant to acid, alkali and corrosion. There are drawers and cabinets under the cabinet to facilitate the placement of laboratory supplies.
2.13. The P2 laboratory should be equipped with a biological safety cabinet, which is mainly used for the main isolation equipment of the biological laboratory to prevent the spread of harmful suspended particles. The protection provided to personnel, environment, and test product samples varies according to their levels. The biosafety cabinet is front-opening, protects staff through vertical airflow, protects samples through multilayer HEPA filtration, and filters exhaust gas to protect the environment.

3. P2 laboratory technical parameters

For cleanliness: there is no requirement. 2. Ventilation frequency: open windows for ventilation, 0.5 per second for hundred grades, 60 times per hour for thousand grades, 30-40 times per hour for ten thousand grades, 15-20 times per hour for hundred thousand grades. It needs to be carried out according to the specifications. design. 3. The pressure difference of the adjacent room with the outdoor direction: no requirement. 4. Temperature ℃: 18~275, relative humidity%: 30~706, noise dB(A): ≤607, illuminance lx: more than 300.

4. Precautions in the laboratory

4.1. During the experiment, the doors and windows of the laboratory must be closed;
4.2. After the end of the experiment every day, a sterilization operation device is required to deal with the pollution that occurs in the experiment;
4.3. The waste of biological materials related to the experiment shall be sterilized before being discarded, and the contaminated equipment shall be subjected to high-pressure treatment before being cleared for use or discarded;
4.4. Eating, smoking and food preservation are prohibited in the laboratory;
4.5. Wear gloves to prevent contamination when operating the recombined body, and wash your hands before leaving the laboratory after the operation;
4.6. In all operations, try to avoid generating aerosols that may cause pollution.
4.7. When removing contaminated items from the laboratory, they must be placed in a firm and leak-proof container and sealed in the laboratory before being shipped out;
4.8. Prevent the entry of non-experimental organisms in the laboratory, such as insects and rodents;
4.9. Wear the lab coat in the laboratory and take it off before leaving;
4.10. When the experiment is in progress, sign “P2 laboratory” at the entrance, and put the sign of “P2 experiment in progress”, and the refrigerators and freezers that store the recombinants should also be marked the same.
4.11 The laboratory should be cleaned frequently and kept clean, and no items unrelated to the experiment should be placed.

1.Laboratory Bench Classified by Material

In order to meet the needs of laboratories with various experimental contents, laboratory furniture should meet the functionality, robustness, corrosion resistance, and flexibility of installation and layout while pursuing a comfortable and safe experimental environment. Laboratory furniture is different from household furniture. Its use is often in contact with water, electricity, gas, chemical substances and materials, as well as instruments and equipment. Therefore, higher requirements are placed on the structure and material of the furniture, and experiments must be paid attention to during laboratory construction. Room furniture design and selection. There is a lot of laboratory furniture, but to sum up, it is mainly a few categories such as laboratory benches, laboratory cabinets and fume hoods!

1. Wood lab bench: This is a commonly used in last 20 years. It is lighter and more flexible, and can be made into various unit combinations. The disadvantage is that the wood consumption is large.
2. Steel lab bench: This structure is composed of steel cabinets, countertops, and reagent racks. The steel cabinet is made of steel with epoxy coating, the feet of the foot bracket is equipped with fine adjustment screws, and there is a pipe space between the steel brackets. The utensil cabinet is a wooden component that can be flexibly hung on a steel bracket. Although this material has anti-corrosion coating on its appearance, it is unavoidable to be rusted, and it is generally used in instrument analysis rooms. The advantages of steel laboratory furniture: sturdy, beautiful, can be used for more than 10 years without deformation, can withstand strong acids and alkalis, which is the development direction of laboratory furniture.
3. Reinforced concrete lab bench: This kind of laboratory bench includes the countertop and support (or brick support) of the reinforced concrete structure and the utensil cabinet under the counter. Its disadvantages are high quality and lack of flexibility.
4. C frame-mounted lab bench: using a steel frame, reinforced concrete countertops or new countertop materials and wooden utensil cabinets with movable lower parts, this may also be made into a unit combination type, which is lighter and better than the previous one in terms of flexibility.

3. Laboratory Bench Classified by Laboratory purpose

There are a wide variety of chemical Laboratory Benches, instrument Laboratory Benches, sky platforms, washing benches, etc. Depending on the layout of the laboratory, the experimental platform is located in the middle of the laboratory and is called an island-style experimental platform; one end of the experimental platform is against the wall is called a peninsula experimental platform; if one long side of the experimental platform is against the wall, it is called a side experimental platform. The corresponding reagent racks, washing stations, and utensil cabinets are also different. Some also divide the experimental platform into a single-sided experimental platform and double-sided experimental platform, the latter actually refers to an island-style experimental platform and peninsula-style experimental platform.

Currently, the most commonly used countertop materials are as follows:

Epoxy resin countertop: It is mainly composed of reinforced epoxy resin, with smooth and safe arc-shaped edges. It is a chemical countertop formed by a mold. The internal and external materials are the same, which can be repaired and restored when damaged. It has excellent acid and alkali resistance, impact resistance, and high-temperature resistance (about 800°C). Corrosion-resistant hardened board: It is made of high-quality multi-jammed paper after the screening, soaked in a special phenol liquid, and shaped by high-pressure thermosetting effect, and has a special corrosion-resistant surface treatment. It has the characteristics of acid and alkali resistance, impact resistance and heat resistance, economical and durable.

TRESPA: 70% wood fiber, 30% melamine resin, double electron beam scanning patent technology to attach melamine to the surface layer, and high temperature and high-pressure molding. The corrosion-resistant physical and chemical veneer is made of kraft paper impregnated with special phenolic resin, white and special surface paper after high temperature and high-pressure treatment. It has the characteristics of acid and alkali resistance, impact resistance and heat resistance. Economical and durable (used with base material).

2. Laboratory storage cabinet

1. Medicine cabinet

The medicine cabinet is an indispensable cabinet in a chemical laboratory. The solid chemical reagents and standard solutions are mainly placed. The two must be placed separately and cannot be mixed together. Chemical reagents should be placed in categories for easy search. At the same time, for safety, the medicine cabinet should be equipped with glass doors and windows, and the cabinet body should also have a certain load-bearing capacity and corrosion resistance.

2. Special purpose cabinet

(1) Sample cabinet: The sample cabinet used to place various experimental samples should have partitions with compartments and labels that can be used to store samples and find samples, because some samples are required according to the physical properties and chemical stability of the samples. Store in a desiccator, so the compartments are large and small to facilitate the storage of different samples.

(2) Drug storage cabinet: used to store liquid reagents, such as hydrochloric acid, nitric acid, perchloric acid, organic reagents and other volatile drugs. It is generally made of wood or steel, which can store reagents of different specifications. The cabinets are required to be stable and can be used side by side or fixed to the ground wall.

(3) Dangerous goods storage cabinet: suitable for simple storage and short-term storage of dangerous goods, made of stainless steel, or made of refractory bricks.

(4) Glassware drying and storage cabinet: The cleaned off-duty utensils are stored on the bracket, and the bracket is fixed to the cabinet with a guide rail to make the glassware easy to access. The position of each layer of the bracket can be adjusted according to the size of the utensil. The utensil rack is well ventilated and easy to clean and dry. In addition, there are tool cabinets, sundries cabinets, and lockers.

(5) Flammable storage cabinet: This cabinet is used to store flammable liquid chemicals. The cabinet should be double-wall construction to improve the fire resistance performance. It is epoxy coated in red, yellow and blue color for storage of different chemicals

3.Lab chair and lab stool

According to laboratories’ needs, the lab chair and stool can be made of PU leather surface or PU foaming surface. To facilitate experiments and save space, round stools are usually used. The height of the round stool can be adjusted if it is made of steel. The instrument laboratory can have a chair with wheels for easy operation.